Beyond the Nucleosome: Nucleosome-Protein Interactions and Higher Order Chromatin Structure

• We propose 7 ways for nucleosome binding proteins to affect higher order structure. Choices on nucleosomal array conditions impact protein and overall Chromatin phase separation is driven by a range of interactions the nucleosome. The regulation chromatin biology ultimately depends manipulation its smallest subunit, that bind operate do so, while their substrate part polymer embedded in dense nuclear environment. Their molecular must some way be tuned deal with this complexity. Due rapid increase number high-resolution structures nucleosome-protein complexes increasing understanding cellular structure, it starting become clearer how factors complex In review, we analyze current literature interplay between higher-order examine what nucleosomes-protein can affected organization at oligonucleosomal level. addition, review characteristics cause chromatin. Throughout, hope illustrate exciting challenges characterizing beyond